@adamezracohen.bsky.social
@zebrafishrock.bsky.socialReposted by @adamezracohen.bsky.socialTo continue our #Inktober & #Spooktober theme, we are highlighting the use of real #zebrafish embryos as our canvas! 🎨🐟🥚 Enjoy these spooky "Fish-O-Lanterns" expressing a photo-activated fluorescent protein 🎃 Credit to @harrymcnamara.bsky.social & @adamezracohen.bsky.social 🤩 #NoAI #ZebrafishZunday
@tonykellyeu.bsky.socialReposted by @adamezracohen.bsky.socialVery happy our work on a weird new nanoscale morphological feature is out in @science.org. A massive effort involving different high resolution microscopy techniques, multiple institutes, & years of work to convince ourselves DENDRITIC NARROWINGS EXIST #neuroskyence 🧪 doi.org/10.1126/scia...
@andrewgyork.bsky.socialReposted by @adamezracohen.bsky.socialReally happy to see this work from the Shapiro lab. New colleagues in an exciting new field!
@adamezracohen.bsky.socialFor in vivo (mouse) imaging of neural firing, we see ~30% bleaching after ~1 h of recording. Depends a lot on imaging intensity, dye, tissue conditions, etc.
@adamezracohen.bsky.socialAnd if you want to buy an ultra wide-field voltage imaging setup, with patterned optogenetic stim: www.luminosmicro.com
@adamezracohen.bsky.socialThe tutorial focuses on practical implementation. Highlights:
an interactive database of voltage indicators: gevibench.org;
open-source instrument-control code for optical electrophysiology: www.luminosmicroscopy.com;
Some particularly useful data analysis scripts: github.com/adamcohenlab...
@adamezracohen.bsky.socialWe wrote a tutorial guide on voltage imaging, now out in Neuron: authors.elsevier.com/a/1nlgh3BtfH...
Indicators, microscopes, and software have all advanced to the point that it's not so hard -- you can now directly watch the electrical activity of neurons in live animals or in culture.
@adamezracohen.bsky.socialHere's another, showing raw voltage throughout the dendritic tree, the virtual reality location of the animal, the mouse-eye view, and the voltage at the neuron's soma.
@adamezracohen.bsky.socialI wrote about the government's attacks on Harvard. Hope this reaches a few persuadable minds.
prosyn.org/PhWi74r?h=Ky...
