Feng Zhang and Guilhem Faure’s teams discovered that naturally nuclease-inactive TIGR-Tas systems use dual-spacer tigRNAs to act as natural RNA-guided transcriptional repressors in E. coli. 🧬🤖 #FengZhang #TIGRTas #CRISPRi #bioRxiv2026 #SyntheticBiology #RNAguided #BroadInstitute