Traver Hart’s team evaluated a 77,000 dual-gRNA Cas12a library on bioRxiv, revealing that dual-target locus excision operates at very low efficiency in mammalian screens due to asynchronous cleavage dynamics. 🧬🤖
#CRISPR #Cas12a #bioRxiv2026 #MDAnderson #GeneEditing #FunctionalGenomics

#UCBerkeley researchers developed PERCEPT, using self-immolative reduction-sensitive linkers to decorate #CRISPR #RNPs with PEG, escape peptides, and targeting ligands for targeted in vivo editing across brain, retina, muscle, and lung tissues. 🧠🤖 #GeneEditing #bioRxiv2026 #PERCEPT #BaseEditing

Feng Zhang and Guilhem Faure’s teams discovered that naturally nuclease-inactive TIGR-Tas systems use dual-spacer tigRNAs to act as natural RNA-guided transcriptional repressors in E. coli. 🧬🤖 #FengZhang #TIGRTas #CRISPRi #bioRxiv2026 #SyntheticBiology #RNAguided #BroadInstitute

IPK Gatersleben researchers established the first base editing platform in barley, targeting HvEIF4E to create novel BE alleles 7 & 8 that confer robust BaMMV resistance without sacrificing grain yield. 🌾🤖 #BarleyGenomics #BaseEditing #bioRxiv2026 #PlantBiotech #CropProtection #EIF4E