New lab paper in @natcomms.nature.com!!! With many replicates in long-read RNA-seq, do you pool reads before calling transcripts (Join & Call) or call per sample and merge (Call & Join)? We tested both with 6 tools, PacBio & ONT. It depends on the tool!
doi.org/10.1038/s414...
Ana Conesa
@anaconesa.bsky.social
Computer scientist at I2SysBio-CSIC. Transcriptomics, long-reads, multiomics, tool developer. Promoter of Green Science. Finally happy!
I will be speaking at the @embo.org Scientists' Forum, back in Croatia after 17 years: Dubrovnik, 28 to 30 Oct. For PhD students, postdocs, and early-stage PIs. Every participant can present a poster. Registration extended to 5 Oct: event.fourwaves.com/eysf
🎉 New paper from our lab in Nature Comms!
We compare 2 strategies—Join & Call vs Call & Join—across PacBio and ONT data, tissues, and 6 lrRNAseq transcript reconstruction tools. Take away:
👉Join & Call 4 novel isoforms
👉Call & Join 4 2 obtain highly replicated signals.
www.nature.com/articles/s41...
Ana Conesa @anaconesa.bsky.social is sharing her scientific journey to a full house at #ECCB2026, from the challenges of being a young PI to the success of BLAST2GO and much more on long read
Deadlines. PhD: GrisolĂa 7–25 Sep, FPU 20 Oct–20 Nov, ACIF 1–21 Dec. Postdoc: la Caixa 23 Sep, APOSTD 2–20 Nov, Juan de la Cierva Nov–Dec, EMBO 22 Jan 2027, MSCA-PF next round ~Sep 2027. We write the application with you: [email protected]
What we build: SQANTI3, SQANTI-SIM, SQANTI-reads and TUSCO for long-read QC and benchmarking; tappAS and IsoAnnot for isoform-level function; acorde and scMaSigPro for single cell; maSigPro, NOISeq, PaintOmics, MultiBaC and MOSim for multi-omics. All open source.
Finishing your PhD, or thinking of starting one? The Conesa Lab (I2SysBio, CSIC–UV, Valencia) hosts PhD students and postdocs on competitive personal fellowships: long-read transcriptomics, single-cell, multi-omics, ML for genomics. Calls close Sep 2026–Apr 2027. → [email protected]
