What are some of the use cases that are enabled by PAIR-Scan's scale and capacity for potency ranking? (7/7)

What about precision? Across PAIR-Scan screens, top-ranked pairs validate as reactive and tested lower-ranked pairs as non-reactive, showing near-perfect separation, so users rarely waste validation effort on false positives. (6/7)

Can PAIR-Scan rank TCRs reactive to the same neoantigen by their potency? Yes ✅, PAIR-Scan enrichment ranks TCRs by their functional killing activity. (5/7)

Proof-of-concept: Can PAIR-Scan identify reactive TCR–neoantigen among 40,000 candidate CD8+ TIL TCR and candidate neoantigen combinations? Yes ✅. (4/7)

As TCR signalling drives cell adhesion & Ca2+ flux, we reasoned Ca2+ flux T-B doublets could inform us on reactive pairs. Furthermore, as duration of Ca2+ flux reflects TCR signal strength, we reasoned that it may be feasible to even rank TCR–antigen pairs by their potency (3/7)