Smart microscopy can begin with smarter sampling.
TIDT-NAS samples densely near the specimen and sparsely farther away. In live HeLa cells, 26 axial images replaced 71 while enabling ~1 Hz 4D refractive-index imaging.
@rt-superes.bsky.social
@rt-superes.bsky.socialSmart microscopy can begin with smarter sampling.
TIDT-NAS samples densely near the specimen and sparsely farther away. In live HeLa cells, 26 axial images replaced 71 while enabling ~1 Hz 4D refractive-index imaging.
@rt-superes.bsky.socialLive lattice-SIM reveals lysosome repair as a spatial event: DFCP1-positive ER microdomains dynamically assemble around damaged lysosomes and help concentrate lipid-transfer machinery for repair.
@rt-superes.bsky.socialMicroscopy reproducibility should include the analysis path, not only acquisition metadata.
As imaging becomes adaptive, decisions that alter what is acquired next should be traceable and reproducible.
@rt-superes.bsky.social“Most informative” is not intrinsic to an image. SimuScan can identify features and steer AFM follow-up scans, but targets are ranked by user-defined criteria. AI can choose where to look; science still defines what matters.
@rt-superes.bsky.socialBiology can disappear when the field of view ends.
WHOLISTIC records cellular Ca²⁺ activity across larval zebrafish. During hypoxia, it revealed brainstem control of blood-flow redistribution between organs.
@rt-superes.bsky.socialWhat if, instead of filtering background away, we moved the signal somewhere the background isn't?
PP-LID makes upconversion nanoparticles generate a beat-frequency signal absent from the excitation itself, enabling camera-based isolation.
@rt-superes.bsky.socialDoes microscopy analysis always need the full image volume?
DeepWonder3D works from multiview projections rather than every voxel, improving 3D neuronal localization with ~10× lower computational cost.
Sometimes the representation is part of the solution.
@rt-superes.bsky.socialWhat if multiple imaging modes could be encoded into the coverslip?
A new preprint demonstrates a meta-coverslip for bright-field, differential, fluorescence and holographic imaging, switching functions simply by changing wavelength.
@rt-superes.bsky.socialSometimes nanometres are not just resolution. They are biology.
A new study shows that membrane-to-cortex distance can regulate mDia1 activity and cell mechanics, making nanoscale geometry itself a functional cellular parameter.