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@devbiol.bsky.social Oct 2, 2026, 1:48 PM

Regenerated fish retinas are similar to undamaged retinas, but display local patterning errors in comparison with undamaged retinas; new models could test their functionality recovery.

By Deborah L. Stenkamp

doi.org/10.1016/j.ydbio.2026.07.009

Examples of lamination and topographical patterning outcomes for regenerated fish retinas. A. Laminar fusions and rosettes can form in retina regenerated after a high dose ouabain lesion (=Extensive). Left panel shows blue-sensitive cones (red fluorescence), PKCα+ bipolar cells (green fluorescence) and nuclei (DAPI, blue fluorescence) in control retinal cryosection. Middle panel shows lamination, with some errors (LF = laminar fusion), of retina regenerated 45 dpi (days post-injury). Scale bar = 50 μm. Right panel shows red- and green-sensitive cones (red fluorescence, Zpr-1+) in retina regenerated 100 dpi (R = rosette). Scale bar = 25 μm. ONL, outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer; s4-6 refers to sublaminae in IPL. B. Local patterns of cones are not restored in retina regenerated after a light lesion. Left panel shows cartoon of cone mosaic organization in zebrafish retina; colors of profiles roughly correspond to λmax of cone subtype. Middle panel shows arrangement of blue-sensitive cones (dark blue color product) in undamaged whole mounted zebrafish retina subjected to in situ hybridization. Right panel shows blue-sensitive cone arrangement in retina regenerated after light lesion. Scale bar = 25 μm. C. Topography of red-sensitive cones (LWS cones) in retina regenerated after high dose ouabain lesion. Left two panels show whole mounted undamaged control retina (Con), and contralateral regenerated retina (Reg), with fluorescent reporters for lws1 (green) and lws2 (magenta). Scale bars = 500 μm. Right two panels show closer views of lws1-reporter + mosaic only in control (Con) and regenerated (Reg) retina. Scale bar = 10 μm. D, dorsal; V, ventral; N, nasal; T, temporal.

@devbiol.bsky.social Sep 30, 2026, 12:45 PM

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Neural crest marker FOXD3 regulates ventralising SHH expression in the floorplate and is required for proper neural tube morphogenesis.

By Jenaid Rees, Laura Kerosuo, et al.

doi.org/10.1016/j.ydbio.2026.07.001

A common gene expression pattern between the neural crest and floorplate. To identify genes in common between the FOXD3-positive cells in the dorsal and ventral neural tube that are not highly expressed in the rest of the neural tube, we selected genes from the list of top 100 markers from our single cell RNA sequencing analysis (of pooled FOXD3-positive cells) based on UMAP distributions reflecting this pattern. This included neural crest marker genes (A) FOXD3, (B) SOX5 and (C) OLFML3, actin and fibronectin binding proteins (D) COTL1 and (E) FBLN1, and GTP-binding proteins (F) DLC1 and (G) ARL4A. (H–N) Fluorescent in situ Hybridization Chain Reaction for these genes on cranial chicken embryo sections at HH9 shows a distinct pattern of increased gene expression in the neural crest and floorplate in comparison to the rest of the neural tube (outlined by grey dashed lines). Scale bar: 25 μm.

@devbiol.bsky.social Sep 28, 2026, 9:30 AM

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A central role for Islr2 in direct pathway striatal projection neurons for the correct formation of the internal capsule and cerebral peduncle

By S Ali, J Ehrman, P Merchan-Sala, M Kofron, R Waclaw, K Campbell

doi.org/10.1016/j.ydbio.2026.07.003

Axonal defects in Isl1cre-vs Foxd1cre-driven Islr2 cKOs. A-L. E18.5 sagittal mouse brain sections of control (A,D,G,J,M Isl1cre;Islr2flx/+; Sox8-EGFP; Fezf2-TdTomato), Isl1cre-driven Islr2 cKO (B,E,H,K,N Isl1cre;Islr2flx/flx; Sox8-EGFP; Fezf2-TdTomato) and Foxd1cre-driven Islr2 cKO (C,F,I,L,O Foxd1cre; Islr2flx/flx; Sox8-EGFP; Fezf2-TdTomato). A-C. Sox8-EGFP labeling of dSPN projections. D-F. Fezf2-TdTomato labeling of corticofugal projections. G-I. Netrin-G1a labeling of TCAs (arrows in G,I show cortical innervation). J-O. Merged images show a severe disruption of dSPN axons, CFAs (arrowhead in B,E,K,N) and TCAs (open arrowhead showing normal telencephalic entry point in G-I, M-O and misrouted axons indicated by closed arrowhead in H) in Isl1cre-driven Islr2 cKO when compared to control (A,D,G,J). In contrast, Foxd1cre -driven Islr2 cKO (C,F,I,L,O) exhibit overtly normal axon trajectories. P-R. Schematics made using bioRender, with Islr2 expressing regions in yellow and recombined regions in white showing dSPN axon (green), CFA (red), and TCA (blue) trajectories in control (P) and Islr2 cKO - either Isl1cre-driven (mutant dSPNs shown with open green arrow; Q) or Foxd1cre-driven (R). cp, cerebral peduncle; Ctx, cortex; GP, globus pallidus; ic, internal capsule; TRN, thalamic reticular nucleus; SNr, substantia nigra pars reticulata; Stm, striatum; STN, subthalamic nucleus; Th, thalamus. Scale bar in A-L = 500 μm; M-O = 125 μm.

@devbiol.bsky.social Sep 21, 2026, 11:56 AM

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Thyroid-hormone-induced metamorphosis has been deregulation in the evolution of obligately paedomorphic salamanders

By Ronald Bonett, Samantha Trame

doi.org/10.1016/j.ydbio.2026.06.009

Head images of the obligately larval-form paedomorphic Three-toed Amphiuma (Amphiuma tridactylum) above and Axolotl (Ambystoma mexicanum) below. Note the loss of external gills but maintenance of a gill slit on A. tridactylum, which is an example of partial metamorphosis. Photos by S. D. Trame and R. M. Bonett respectively.

@devbiol.bsky.social Sep 17, 2026, 7:09 AM

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The oocytes of basal dermapterans (earwigs) lack the posterior pole lysosomal compartment

By Szczepan Bilinski, Magda Sochaczewska, Paula Irles, Stanislaw Miodonski, Andres Sarrazin, Waclaw Tworzydlo

doi.org/10.1016/j.ydbio.2026.07.002

Distribution of lysosome-like bodies in the oocytes of basal and derived dermapterans. In basal dermapterans, lysosome-like bodies remain dispersed throughout oogenesis, whereas in eudermapterans they accumulate at the posterior pole of the oocyte to form the posterior pole lysosomal compartment (PPLC). Families analyzed in the present study are highlighted in red (Pygidicranidae: Gonolabina spectabilis; Anisolabididae: Euborellia annulipes). The family analyzed in the parallel study (Sochaczewska et al., 2026; this issue) is highlighted in blue (Forficulidae: Apterygida media and Forficula auricularia).

@devbiol.bsky.social Sep 11, 2026, 11:40 AM

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Branch extension and intracellular lumen formation are spatio-temporally coupled throughout Drosophila larval stages, and nascent tubes are initially fluid-filled

By Tanner Simpson, Jodi Schottenfeld-Roames

doi.org/10.1016/j.ydbio.2026.07.010

Terminal cells grow but do not form new branches or nascent fluid-filled tubes during the final larval instar. (E) Cartoon model of the entire late L3 terminal cell (gray) shown in F-H. The boxed regions in magenta are magnified in F-H’. (F-H′) Composite images of terminal branches (membrane in magenta) located at the anterior (F-F′), central (G-G′), and posterior (H-H′) regions of the terminal cell show accumulations of Lum:GFP (green) at the distal tips of tubes (F′, G′, H′). All tubes in this cell are gas-filled to the point of this Lum:GFP (data not shown). All scale bars represent 10 μm. Scale bar in A applies to A′ and scale bar in F applies to F-H’.

@devbiol.bsky.social Sep 9, 2026, 9:32 AM

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Drosophila eye development is compromised by dTet overexpression through altered retinal determination gene expression

By Malak Kleit, Sarah Mantash, Abdullah El-Kurdi, Jawdat Sandakly, Margret Shirinian, et al

doi.org/10.1016/j.ydbio.2026.07.006

Overexpression of dTet in eyes leads to total or partial loss of eye imaginal discs. Immunofluorescence staining for Elav, a pan-neuronal RNA-binding protein, was performed on eye imaginal discs from control, dTet-overexpressing, and dTet knockdown flies. Scale bar, 20 μm and 50 μm.

@devbiol.bsky.social Sep 7, 2026, 12:14 PM

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SFRP1 loss reveals mesenchymal mechanisms underlying abnormal lung maturation via Wnt and PDGF signaling

By Nooralam Rai, Tina Zelonina, Kiran Chada, Jeanine D'Armiento

doi.org/10.1016/j.ydbio.2026.06.011