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@sicbjournals.bsky.social Oct 1, 2026, 11:38 AM

ICB Throwback Axolotl Thursday

#Morphological, #Biochemical and #Immunohistochemical Studies on Heart Development in Cardiac Mutant #Axolotls, Ambystoma mexicanum

LARRY F. LEMANSKI

doi.org/10.1093/icb/...

#amphibians #science

In this paper I describe morphological, biochemical and immunofluorescence studies which compare the contractile proteins in normal and mutant hearts. In addition, morphological studies on anterior endoderm, an important heart inductor tissue in salamanders, are reviewed. Detailed electron microscopic studies show that normal heart myocytes contain numerous well-organized myofibrils. Although mutant heart cells contain a few myosin and actin filaments, there are no organized myofibrils. Instead, amorphous proteinaceous collections are prominent in the peripheral cytoplasm of the cell where myofibrils would be expected to first form. SDS-polyacrylamide gel electrophoresis shows that actin is present in almost normal amounts in mutant hearts, myosin heavy chain is reduced and tropomyosin is virtually absent. #Immunofluorescence studies reveal that myosin, α-actinin and tropomyosin are located prominently in theorganized myofibrils of normal heart cells. In mutant hearts myosin is localized almost exclusively in the amorphous collections at the cell peripheries, α-actinin also is distributed mainly in the peripheral cell cytoplasm.

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