Ana was pretty happy with this one.

Glycine receptor alpha1 homomer, lovely long clusters of openings at 1 mM.

We do theory all morning but in the afternoons it’s full-on wet lab practicals.

#singlechannels #neuroscience
#BSMBC

And that’s that. The 3rd edition of #BSMBC is done. We ended with some analysis examples and software demonstrations.

Many thanks to course faculty @ewerslab.bsky.social Max Ulbrich, Jelena Baranovic and Claudio Grosman, our students, and our super coordinator Dr Sandra Keiper, without whom🙏❤️

One of the most beautiful experiments ever!

Single proton pKa measurements from the nicotinic receptor, Claudio Grosman’s masterpiece.

#BSMBC

www.nature.com/articles/nat...

Now part two.

No colour but incredible details from a giant of the field. Claudio Grosman outlines his pioneering work on reaction mechanisms via single channel recording.

#BSMBC

At the Berlin Single Molecule #biophysics course #BSMBC every participant also gives a 5 minute “chalk talk” on their own work. This is a great way to bring everyone into the discussion, and to make new connections.

Here Shahid Iqbal from Bergen told us about his work on ion channel receptors.

How it started…how it’s going.

At the start of the week, a pile of parts.

Jelena & I threw the minipatch together in the lunch break.

By Tuesday, we used it for nice cell-attached patch recordings.

Students (Nanna & David) learn about grounding.

The recording “box” is bottom right.

#BSMBC

We are immensely grateful to Max Ulbrich from Freiburg, who transformed the imaging quality on our live cell #TIRF setup! Max also gave a wonderful lecture on his work counting subunits.

#BSMBC

Jelena Baranovic from the University of Edinburgh told us about her work on #iglurs and single channels.

She also got several sets of students to record single glycine receptors.

#BSMBC

@ewerslab.bsky.social showed the students single molecule TIRF!

#BSMBC

We’ve had a great week with single molecules at the HU Berlin on the #BSMBC . Fantastic students, great spirit.

Today we wrap up with lectures from two of the best in the business.
First, we are honoured to have @ewerslab.bsky.social here to tell us about single molecule localisation microscopy.