(BioRxiv All) Site-resolved spatial and structural interactome of a human cell: The spatial and structural arrangement of proteins determine virtually every process in human cells. We combined gentle subcellular fractionation by differential ultracentrifugation with… #BioRxiv #MassSpecRSS

(BioRxiv All) AlphaPeptTools: scverse-native analysis of mass spectrometry-based proteomics: Mass spectrometry (MS)-based proteomics now routinely profiles proteomes at scale, but extracting biological insight and integrating complementary modalities remains challenging. Here,… #BioRxiv #MassSpecRSS

(BioRxiv All) Zoospore-derived extracellular vesicles in the flagellated stramenopile Phytophthora parasitica: Zoospores are unicellular, wall-less and flagellated cells produced by a number of eukaryotic microorganisms. They allow microbial dispersion, enable the search and… #BioRxiv #MassSpecRSS

(BioRxiv All) Deoxyribonucleotide dephosphorylation by VENOSA4 supports organellar genome replication in plants: The replication of the three genomes in plant cells during germination is a complex process, which requires a high degree of coordination between the… #BioRxiv #MassSpecRSS

(BioRxiv All) Adjunctive nebulization of Idursulfase to intravenous treatment enhances cardiac proteome adaptations in IDS-KO mice: Mucopolysaccharidosis Type II (MPS-II) is a rare X-linked lysosomal storage disorder, characterized by deficiency of the lysosomal hydrolase… #BioRxiv #MassSpecRSS

(BioRxiv All) The PSD-95 interactome of hippocampal synapses potentiated in response to an in vivo learning task: Changes in the molecular composition of synapses underlie the functional changes in synaptic strength after learning. These phenomena have been studied either via… #BioRxiv #MassSpecRSS

(BioRxiv All) Nucleocytoplasmic shuttling of the herpesvirus tegument protein UL47_TEG5 is required for its functional role in horizontal transmission in chickens: Mareks disease virus (MDV) UL47 tegument protein 5 (UL47_TEG5) is a major tegument protein essential for viral… #BioRxiv #MassSpecRSS

(BioRxiv All) Proteomic and Kinetic Analyses Reveal Discordant Apolipoprotein Turnover and Support a Revised Model of Human Lipoprotein(a) Metabolism: Objective: Lipoprotein(a) [Lp(a)] is a causal risk factor for atherosclerotic cardiovascular disease composed of… #BioRxiv #MassSpecRSS

(BioRxiv All) Arrayed hydrogels pair whole-cell imaging with single-cell mass spectrometry proteomics: Multimodal single-cell analysis aims to elucidate cellular-level phenotype within heterogeneous cell populations. Advancements in single-cell proteomics (SCP) seek to deepen… #BioRxiv #MassSpecRSS

(BioRxiv All) A Synthetic Archaeal Lipid Analog Enables Quantification of Substrate, Products, and Intermediates in the Tetraether Synthase Reaction: Glycerol dibiphytanyl glycerol tetraether (GDGT) and macrocyclic diether lipids (mAG) are unique membrane lipids predominantly… #BioRxiv #MassSpecRSS

(BioRxiv All) Allosteric remodelling of the Ebola virus glycoprotein underlies differences in entry mechanisms across species: During Ebolavirus transit through the endo-lysosomal pathway, the glycoprotein GP undergoes a series of conformational rearrangements upon cathepsin… #BioRxiv #MassSpecRSS

(BioRxiv All) Polyploid Achromatium sp. expresses protein variants based on environmental cues: Achromatium spp. are giant bacteria harboring hundreds of non-clonal chromosomes per cell. The allelic divergence they exhibit could be a mechanism to enable fine-tuned responses to… #BioRxiv #MassSpecRSS

(BioRxiv All) Native Hydrogen/Deuterium Exchange Ion Mobility Mass Spectrometry of Structured DNA Oligonucleotides: Hydrogen/deuterium exchange coupled to mass spectrometry (HDX/MS) is a powerful technique to probe nucleic acid secondary structures and dynamics, but its… #BioRxiv #MassSpecRSS

(BioRxiv All) Exosome-Mediated Bidirectional Signaling Regulates Glioma Stem-Cell Homeostasis and Radiation-Induced Plasticity: Background: Glioblastoma (GBM) is an aggressive brain malignancy characterized by therapeutic resistance and frequent recurrence. Glioma-initiating… #BioRxiv #MassSpecRSS

Did you know that @biorxiv-medrxiv.bsky.social posts weekly to share updates and features that can help you make the most out of your preprint? Follow us there to learn how to leverage preprints to their fullest potential. #preprints #openscience #openrxiv #biorxiv #medrxiv

(BioRxiv All) Spatial lipidomics suggests fatty acid scavenging by an intranuclear parasite: Bacteria of the family Endozoicomonadaceae are widely associated with marine animals, yet their in situ metabolism remains mostly unknown. Genomic studies suggest that members of this… #BioRxiv #MassSpecRSS

(BioRxiv All) Learning from tandem mass spectra at scale with a self-supervised foundation model for proteomics: Mass spectrometry-based proteomics increasingly relies on machine learning, yet existing models are trained for defined supervised tasks such as peptide… #BioRxiv #MassSpecRSS

(BioRxiv All) Molecular architecture and assembly of the human RNA Degradosome: The PNPase exoribonuclease interacts with the SUV3 helicase to form the human RNA Degradosome responsible for processive mitochondrial RNA turnover and quality control. Although structural data… #BioRxiv #MassSpecRSS

(BioRxiv All) Deep visual proteomics reveals distinct proximal tubular and glomerular injury programs in experimental diabetic kidney disease: Background: Diabetic kidney disease (DKD) is the leading cause of chronic kidney disease (CKD). However, most proteomic studies of DKD… #BioRxiv #MassSpecRSS

(BioRxiv All) Interactive downstream proteomics analysis with MiraProt using Mueller cell proteomes from equine recurrent uveitis: Mass spectrometry-based proteomics requires downstream analysis of processed protein abundance data, including data inspection, filtering,… #BioRxiv #MassSpecRSS