I can't overstate the importance of this toolbox & this paper! Really amazing stuff

6️⃣ There are a few other fun things in the supplement, including the mapping of other aquaporins and comparisons with MRI measurements of the glymphatic system (intrathecal CSF tracer gadobutrol).

5️⃣ Finally, we show that peritumoral edema is most frequent in AQP4-enriched regions and is further shaped by regional inflammatory tone.

4️⃣ Incorporating normative PET markers of neuroinflammation typically strengthens the spatial alignment between AQP4 expression and disease atrophy, suggesting that inflammatory tone and glymphatic-related architecture jointly shape vulnerability.

3️⃣ Turning to neurodegeneration, we find that atrophy patterns across multiple neurodegenerative diseases co-localize with AQP4 expression—most strongly for tau and TDP-43 proteinopathies—and high-atrophy regions lie close to AQP4 hotspots.

2️⃣ Linking AQP4 expression to vascular organization, we find that AQP4-enriched regions show lower normative blood perfusion and lower vein density, suggesting that this axis is not simply explained by vascular supply or large-vessel anatomy.

1️⃣ Aquaporin-4 (AQP4) water channels support the glymphatic system, a brain-wide pathway that clears cerebral waste products. Here we use AQP4 gene expression as a molecular anchor to reconstruct a whole-brain glymphatic-related topography.

3️⃣ We showcase three walkthroughs of biologically-relevant questions that can be answered using netneurotools, related to structure-function coupling, multimodal connectivity, dynamics and gradients.

2️⃣ By design, netneurotools is trainee-centric and focuses on accessibility and usability. The functions are grouped into intuitive modules, covering the full lifecycle of a project. Like "desire paths" in urban design — we only pave the routes people are already walking.