@humollidorentina.bsky.social
PhD student studying phage-host interactions at ETH Zürich | Lab of Molecular Phage Biology
@humollidorentina.bsky.socialWe wrote a short “Behind the Paper” post about our HIDEN-SEQ journey together with @aharms485.bsky.social.
Check it out: go.nature.com/4yBHsdy
Special to share it with the @natmicrobiol.nature.com cover, featuring the amazing art of the great scientist and artist Sophia Zimmermann from our lab!
@humollidorentina.bsky.socialThank you!
@humollidorentina.bsky.socialThank you! Excited to see more people use it🧬
@humollidorentina.bsky.social🧵
@humollidorentina.bsky.socialPhage TnSeq approaches seem to be broadly applicable across different phage-host systems. See parallel work from two other labs:
@peterfineran.bsky.social - www.nature.com/articles/s41...
@jbdsf.bsky.social - www.sciencedirect.com/science/arti...
@humollidorentina.bsky.socialVery grateful to @aharms485.bsky.social for the incredible support along the way. Huge thanks also to all the co-authors for their contributions @damienpiel.bsky.social, Jessica Ransome, @veeninglab.com and other collaborators from @nccr-antiresist.bsky.social
@humollidorentina.bsky.socialWant to try it on your favorite E. coli phage? The HIDEN-SEQ plasmids are available through Addgene!
@humollidorentina.bsky.socialVery happy to share that HIDEN-SEQ is now out in @natmicrobiol.nature.com!
Using this technique, we recapitulated decades of established phage genetics and uncovered new gene functions, including previously unknown anti-defense factors against several defense systems.
www.nature.com/articles/s41...
@vivekmutalik.bsky.socialReposted by @humollidorentina.bsky.socialPreprint here: Please let us know if we have missed any references to cite. This is like looking at past 60 years of work with a small window.
www.biorxiv.org/content/10.6...
@doudna-lab.bsky.socialReposted by @humollidorentina.bsky.socialOut now in Science: Two papers from our lab reveal VIPR, an RNA-guided DNA-targeting system in bacteriophages, providing clues to the origins of CRISPR
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Congrats to Peter Yoon, @kenjmloi.bsky.social @terryzzy.bsky.social, T.Docter & team
