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Solid Evidence

@solidevidence.bsky.social

34 Following9.4k Followers

Molecular virologist, sewage sage and wastewater wizard.

https://lung.fish

Marc Johnson
Professor

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@solidevidence.bsky.socialSep 24, 2026, 1:14 PM

Working on it.

@solidevidence.bsky.socialSep 24, 2026, 1:13 PM

13 states, ~30 million people. East to West.

@solidevidence.bsky.socialSep 21, 2026, 7:51 PM

I stopped counting because the number kept going up.

@solidevidence.bsky.socialSep 21, 2026, 7:44 PM

Every week I get a metagenomic readout of the most elevated viruses from wastewater collected the week before (44 sites). COVID was on the list today for the first time since last winter, along with D68 and a bunch of rhinoviruses.

@solidevidence.bsky.socialSep 17, 2026, 5:56 PM

The good news is that I have not noted its spread correlating with increases in total COVID signal.

@solidevidence.bsky.socialSep 17, 2026, 5:55 PM

All I can say is that the sequences were all homogeneous. Most of the time there are several lineages present, but this sample had only BA.3.2, and the RBD reads all had those 3 extra changes.

@solidevidence.bsky.socialSep 16, 2026, 5:38 PM

I meant PJ.2.1

@solidevidence.bsky.socialSep 16, 2026, 4:43 PM

Even more from the G24077A+T24079A screen.

@solidevidence.bsky.socialSep 16, 2026, 4:43 PM

PJ.3.2 seems to be hitting the accelerator. These are the wastewater samples deposited in just the last week where it was present (based on 439K marker).

@solidevidence.bsky.socialSep 16, 2026, 4:34 PM

WTH, I think I found a BA.3.2 cryptic (I hope it isn't circulating anyway).

Standard RBD + A435F, S438F, P463F.

Wonder if that improves ACE2 binding?

It was 100% of the sequence from one sewershed.
@ryanhisner.bsky.social

@solidevidence.bsky.socialSep 11, 2026, 6:30 PM

I know there were groups that looked at this in mice, but I don't think there was any meaningful immunity.

@solidevidence.bsky.socialSep 11, 2026, 12:42 AM

As long as you don't think about it...

@solidevidence.bsky.socialSep 10, 2026, 6:28 PM

The relevant people were notified, yes.

@solidevidence.bsky.socialSep 10, 2026, 6:27 PM

Correct. No infectious virus.

@solidevidence.bsky.socialSep 10, 2026, 3:01 PM

I picked up a carton of milk from the store this week that had H5N1 influenza in it. >150 million copies/liter! Thankfully, it was safe to drink because the milk was pasteurized, so I’m not saying what brand. Takehome: DON’T DRINK RAW MILK.

@solidevidence.bsky.socialSep 4, 2026, 4:00 PM

Sure, pretty easy if you have the samples

@solidevidence.bsky.socialSep 4, 2026, 3:58 PM

Sorry, missed this. It was down briefly but I think it was fixed.

@solidevidence.bsky.socialAug 22, 2026, 12:37 PM

Update. I was wrong, the sequence was normal. They just got a one-off really high sample (a hot turd). It happens.

SSolid Evidence@solidevidence.bsky.socialAug 18, 2026, 12:37 PM

There have been hints of a cryptic lineage from Anchorage for years. Who thinks they finally popped? bsky.app/profile/soli...

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@solidevidence.bsky.socialAug 20, 2026, 3:45 PM

I can finally reveal the rest of our World Cup surveillance.

While we were working away on wastewater metagenomics, the other side of lung.fish was on the ground doing air sampling with team Canada.

Preprint just out.

www.medrxiv.org/content/10.6...

lung.fishLungfishDedicated to improving environmental surveillance and tackling viruses through innovative detection strategies.
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@solidevidence.bsky.socialAug 14, 2026, 3:19 PM

As you may know, we maintain a public dashboard about the species detected from wastewater samples.

Why do we do this? This is part of an NIH-funded project to monitor viral zoonosis.
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dholab.github.io/public_viz/0...

dholab.github.ioComposition of Genetic Material in Wastewater
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