Cool story on the importance of feedback loop and a phase transition in co-culture growth on chitin with connected modeling

Cool story on the importance of feedback loop and a phase transition in co-culture growth on chitin with connected modeling
Uwe now switches to a stable 10-member course gut community (from OMM12 strains) and the influence of different polymers
and back to marine systems and chitin particles (spatial restrictions) through the PNAS paper of @sammy-pontrelli.bsky.social
www.pnas.org/doi/10.1073/...
Uwe Sauer on Metabolic cross-feeding networks in gut and ocean communities
First, explaining their work on assembly of chitin degrading communities, see in Science Advances by @sammy-pontrelli.bsky.social et al
www.science.org/doi/10.1126/...
Flora explains their work using a "Advanced Mobile Laboratory" to find and analyze diatom directly from sample site, catching symbiosis life cycle
See about the advanced mobile lab
youtu.be/8lVeuH8GpRc?...
@floravincent.bsky.social on symbiosis in marine unicellular eukaryotes - online presentation at #MibiNet
first on diatom cell structure, see their @currentbiology.bsky.social
www.cell.com/current-biol...
Sarahi explains their work on microbial diversity in enrichment pure cultures - the value of community-based cultivation to discover different ecological strategies, autotrophy for amino acids and vitamins
See their work in @commsbio.nature.com
www.nature.com/articles/s42...
Sarahi highlights the biological organization concepts applied for microorganisms from cell, population, and pairwise interactions to consortium, microbial cohort, and finally to community level
See their ISME Communications paper
academic.oup.com/ismecommun/a...
Sarahi Garcia @sarilog.bsky.social on Microbial interactions across levels of biological organization
#MibiNet
Caroline explains AMF and the impact on plant development, e.g. how plant regulates the symbiosis, lipid biosynthesis
See their @elife.bsky.social from 2017
elifesciences.org/articles/29107
Caroline Gutjahr @carogutj.bsky.social on nutrient exchange and manipulation in arbuscular mycorrhiza symbiosis
#MibiNet
James explains that their mother machines, growing the wild type and clock mutant next to each other in the mother machines and they observed a minor difference in growth at the first 12 hour which translates to fitness difference
James finishes with highlighting the importance of clock on fitness of Synecococcus
Refers to Woelfle et al 2004 and Hellweger 2010 - fitness caused by clock deletion could be measured in competition in flask, but not at single cell level
James explains their experiments with mother machines (microfluidic devices) to follow gene expression at single cell level for circadian clock expression: removing input had hardly influence, unlike core clock mutants
See their @natcomms.nature.com paper
www.nature.com/articles/s41...
James explain how a simple clock can regulate more complex processes in the cells that are not happening every 24 hours
See their @currentbiology.bsky.social publication
www.cell.com/current-biol...
James introduces circadian clock from humans and animals to plants and focuses on cyanobacterial clock, KaiA, KaiB, and KaiC including the clock in the test tube
PsigC-yfp to follow the robust clock output in Synecococcus at single cell level
See link.springer.com/article/10.1...
James Locke on Dynamic control and robust timing in cyanobacteria
#MibiNet #ChronoSky
Christian describes the work of @ghadayousif.bsky.social in collaboration with @kiranrpatil.bsky.social published recently in @natmicrobiol.nature.com how abundant are cross-feeding of metabolites in soil microbial communities
www.nature.com/articles/s41...
Auxotrophies are everywhere!
Christian explains that metabolic exchanges are common in natural microbial communities
See their work with @kiranrpatil.bsky.social @sarilog.bsky.social @jfriedman.bsky.social and Markus Ralser
www.nature.com/articles/s41...
Christian Kost @kostchristian.bsky.social starts the second day of #MibiNet conference with his talk on Raiding or trading: disentangling metabolic interaction networks of bacteria
#CrossFeeding